Three-dimensional nuclear magnetic resonance spectroscopy for the assignment of protein structure with nitrogen, carbon and hydrogen dimensions.
Notes: - the magnetization is transferred from \(\ce{^{1}H}\) to \(\ce{^{15}N}\) and then via the \(\ce{N-C\upalpha}\ \rm{J}\mbox{-}\rm{coupling}\) to the \(\ce{^{13}C\upalpha}\). From there it is transferred to the \(\ce{^{13}CO}\) via the \(\ce{^{13}C\upalpha}\mbox{-}\ce{^{13}CO}\ \rm{J}\mbox{-}\rm{coupling}\). For detection the magnetization is transferred back the same way: from \(\ce{^{13}CO}\) to \(\ce{^{13}C\upalpha}\), \(\ce{^{15}N}\) and finally \(\ce{^{1}H}\).
- Each \(\ce{NH}\) group will show correlations to both their own and adjacent carbonyls group.
Source:
PAC, 2021, 93, 647. 'Glossary of methods and terms used in analytical spectroscopy (IUPAC Recommendations 2019)' on page 692 (https://doi.org/10.1515/pac-2019-0203)