Term: hold-up volume
https://doi.org/10.1351/goldbook.H02832

Definition:
The volume of eluent required to elute a component, the concentration of which in the stationary phase is negligible compared to that in the mobile phase.

Related Terms:
1) eluent (http://doi.org/10.1351/goldbook.E02040).
2) elute (http://doi.org/10.1351/goldbook.E02041).
3) stationary phase (<em>in chromatography</em>) (http://doi.org/10.1351/goldbook.S05949).
4) mobile phase (<em>in chromatography</em>) (<em>in chromatography</em>) (http://doi.org/10.1351/goldbook.M03952).

Source: Orange Book, 2nd ed., p. 100 (https://media.iupac.org/publications/analytical_compendium/)

Citation: 'hold-up volume' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. https://doi.org/10.1351/goldbook.H02832

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