Term: restriction enzymes
https://doi.org/10.1351/goldbook.R05350

Definition:
Endonucleases which recognize specific base sequences within a DNA helix, creating a double-strand break of DNA. Type I restriction enzymes bind to these recognition sites but subsequently cut the DNA at different sites. Type II restriction enzymes both bind and cut within their recognition or target sites.

Related Terms:
1) dna (http://doi.org/10.1351/goldbook.D01597).
2) recognition sites (http://doi.org/10.1351/goldbook.R05199).
3) helix (http://doi.org/10.1351/goldbook.H02769).
4) enzymes (http://doi.org/10.1351/goldbook.E02159).

Source: PAC, 1992, 64, 143. 'Glossary for chemists of terms used in biotechnology (IUPAC Recommendations 1992)' on page 164 (https://doi.org/10.1351/pac199264010143)

Citation: 'restriction enzymes' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. https://doi.org/10.1351/goldbook.R05350

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