Three-dimensional nuclear magnetic resonance spectroscopy for the assignment of protein structure with one carbon and two hydrogen dimensions.
Notes: - In the initial HMQC step, magnetization is transferred from \(\ce{^{1}H}\) to \(\ce{^{13}C}\) and back again. This is then followed by a NOESY step in which the magnetization is transferred to any other hydrogen nucleus close by before detection.
- The technique requires \(\ce{^{13}C}\) labelling.
- The spectrum is used to obtain restraints for structure calculations.
Source:
PAC, 2021, 93, 647. 'Glossary of methods and terms used in analytical spectroscopy (IUPAC Recommendations 2019)' on page 680 (https://doi.org/10.1515/pac-2019-0203)