Three-dimensional nuclear magnetic resonance spectroscopy for the assignment of protein structure with nitrogen, carbon and hydrogen dimensions.
Note: Magnetization is transferred from
\(\ce{^{1}H\upalpha}\) and
\(\ce{^{1}H\upbeta}\) to
\(\ce{^{13}C\upalpha}\) and
\(\ce{^{13}C\upbeta}\), respectively, and then from
\(\ce{^{13}C\upbeta}\) to
\(\ce{^{13}C\upalpha}\). From here it is transferred first to
\(\ce{^{13}CO}\), then to
\(\ce{^{15}NH}\) and then to
\(\ce{^{1}HN}\) for detection. Along with
CBCANH and
HSQC this forms the standard set of experiments needed for backbone assignment.
Source:
PAC, 2021, 93, 647. 'Glossary of methods and terms used in analytical spectroscopy (IUPAC Recommendations 2019)' on page 682 (https://doi.org/10.1515/pac-2019-0203)