https://doi.org/10.1351/goldbook.M03892
The dependence of an initial where is the observed initial rate, is its limiting value at substrate ), and the substrate concentration when . The definition is experimental, i.e. it applies to any reaction that follows an equation of this general form. The symbols or are sometimes used for . The parameters and (the ' vs. (a ' vs. ('Eadie–Hofstee plot'). A Michaelis–Menten equation is also applicable to the condition where E is present in large excess, in which case the concentration appears in the equation instead of . The term has sometimes been used to describe reactions that proceed according to the scheme: in which case (Briggs–Haldane conditions). It has more usually been applied only to the special case in which and ; in this case is a true
rate of reaction
upon the concentration of a substrate
S that is present in large excess over the concentration of an enzyme or other catalyst
(or reagent
) E with the appearance of saturation
behaviour following the Michaelis-Menten equation: saturation
(i.e. Michaelis constant
') of the equation can be evaluated from the slope and intercept of a linear plot of Lineweaver–Burk plot
') or from slope and intercept of a linear plot of dissociation
constant (Michaelis–Menten conditions).See also:
rate-determining step