https://doi.org/10.1351/goldbook.P04465
The separation of two peaks in terms of their average peak width at base (tR2>tR1): Rs=tR2tR1wb1+wb22=2 (tR2tR1)wb1+wb2 In the case of two adjacent peaks it may be assumed that wb1wb2, and thus, the width of the second peak may be substituted for the average value: RstR2tR1wb2
Sources:
Orange Book, 2nd ed., p. 108 [Terms] [Book]
PAC, 1993, 65, 819. (Nomenclature for chromatography (IUPAC Recommendations 1993)) on page 847 [Terms] [Paper]