https://doi.org/10.1351/goldbook.08347
Three-dimensional nuclear magnetic resonance spectroscopy for the assignment of protein structure with one nitrogen, and two hydrogen dimensions.
Notes:
- Magnetization is transferred from the side-chain hydrogen nuclei to their attached
nuclei. Then isotropic mixing is used to transfer magnetization between the carbon nuclei. From here, magnetization is transferred to the carbonyl carbon, on to the amide nitrogen and finally the amide hydrogen for detection. - Requires
and labelling. - Useful spectrum for obtaining hydrogen side-chain assignments.